Sample Submission

Important: We do not accept any radioactive or biohazardous samples. You will be ask to sign a declaration that your samples are not dangerous when you bring them to the core. You must discuss your experiment with core staff if radioactive materials have been used at any point, even if you do not believe your samples are radioactive.

 

  1. Setup an iLab Account if you do not already have one.
  2. Carefully review the advice relevant to your submission in the sections below.
  3. Prepare gel-bands according to our guidelines, or follow our instructions for in-solution submissions. ALWAYS stain gels – if running a mixture into the top of a gel stain with Coomassie blue. ALWAYS dice gel bands into ~1mm cubes, bands that are not diced WILL NOT be accepted. In-Solution digested samples are subject to restrictions – read the provided information thoroughly.
  4. Include a subledger number with your submission, either with your online submission, by email, or when you drop off your samples.
  5. Login to iLab, our sample submission system  using your UT-Southwestern login info (external customers can create an account through the iLab site) and create a new service job.
  6. Label all sample tubes with the Sample ID created in the submission system. Do not tape around tubes.
  7. Bring your sample to the core lab in Y4.306, with the completed IDR. 9-6pm Mon-Fri.
Schedule / Turn-around

The UTSW core provides a quick turn-around compared to other academic and commercial facilities. For samples submitted no later than 5pm Wednesday we aim to:

  • Prepare samples for mass-spec analysis during Thursday & Friday
  • Run samples on the mass-spectrometer beginning Friday evening
  • Analyze data and supply results through Monday & Tuesday the following week, subject to instrument problems, length of the sample queue etc.
  • TMT samples require a month for analysis due to the additional sample preparation steps and data analysis required.

Samples submitted after 5pm Wednesday will be stored for preparation and analysis the following weekGenerally, if samples are submitted by 5pm Wednesday you will receive results by Wednesday the following week. In busy times, or in the event of instrument problems analysis may require an additional week.

General Guidelines & Information

The following guidance and information applies to all services. If you are a new user please review the information below before submitting your first samples.

  • Preparing Gels & Avoiding Keratin / Other Contamination
  • General Limitations of Proteomics Services
  • Frequently Asked Questions (FAQs)

Service Specific Guidelines & Information

We provide specific information and guidelines for each of the services we offer. Please review the relevant guidance before you submit your samples – it may be updated as we work to improve protocols and widen the range of analyses we offer.

  • Gel Band Protein ID
  • PTM Identification
  • Complex Mixture Identification & Label-Free Quantitation
  • SILAC Quantitation
  • TMT Quantitation
  • In Solution Digested Samples
  • Intact Mass Analysis

Experiment Specific Guidelines & Information

The vast majority of samples we analyze are generated from similar types of experiments occurring across many labs at UTSW. Here we provide advice about how to best make use of the services the core provides for the type of experiment you are performing.

  • Identification of Protein Interactors / Complexes Using Co-Immunoprecipitation (Co-IP)
  • PTM Mapping of a Protein

 


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