{"id":1255,"date":"2017-04-12T09:46:40","date_gmt":"2017-04-12T14:46:40","guid":{"rendered":"https:\/\/proteomics.swmed.edu\/wordpress\/?page_id=1255"},"modified":"2025-04-30T09:56:18","modified_gmt":"2025-04-30T14:56:18","slug":"tmt-quantitation","status":"publish","type":"page","link":"https:\/\/proteomics.swmed.edu\/?page_id=1255","title":{"rendered":"TMT Quantitation"},"content":{"rendered":"

Introduction<\/strong><\/span><\/p>\n

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\"TMT\"<\/p>\n

Tandem Mass Tag (TMT) technology is a powerful technique for comparing protein (and peptide) amounts between six to eighteen conditions. This technique involves labeling samples after digestion, meaning this technique can be used on in vivo<\/em>\u00a0samples, unlike SILAC.<\/p>\n

TMT or TMTpro samples are to be submitted in solution or on-bead, not in gel. It is the responsibility of the customer to ensure that similar amounts of protein is present in each sample and that the samples are contaminant free. A full description of TMT is outside the scope of this website, but details can be found at:<\/p>\n

https:\/\/en.wikipedia.org\/wiki\/Tandem_mass_tag<\/a>
\nhttps:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-mass-spectrometry-analysis\/protein-quantitation-mass-spectrometry\/tandem-mass-tag-systems.html<\/a><\/p>\n

Samples to be analyzed by TMT quantitation should be submitted either on-bead (if applicable), or as in-solution samples in suitable buffer (RIPA buffer or 50 mM triethylammonium bicarbonate and 5% SDS. Other buffers may be suitable, please contact us with questions). Analysis of acquired mass-spectrometry data will be performed with Proteome Discoverer 3.0 software.<\/p>\n

What Does it Cost?<\/strong><\/span><\/p>\n

$1530 for TMT 6-plex, $2040 for TMT 10-plex, $3672 for TMTpro 18-plex. Any number of samples between 2-18 can be sumbitted in a single batch, and prices will be adjusted for labels that are not used. High-pH fractionation is an additional $1,020 per set of up to 18 samples and can significantly increase the number of proteins identified in complex samples.<\/p>\n

What We Do<\/strong><\/span><\/p>\n

We run your TMT\/TMTpro samples on our Orbitrap Eclipse mass spectrometer platform, using an appropriate LC-MS\/MS method including real-time search. We identify and quantify peptides and proteins from samples using Proteome Discoverer 3.0 and send you a spreadsheet including a list of proteins present and the relative amount of each protein in each sample.<\/p>\n

What You Must Do<\/strong><\/span><\/p>\n

Prior to submitting TMT\/TMTpro samples you should:<\/p>\n