Following publication a cell-line contamination error was discovered in a subset of the data used to build Confetti. SAX fractionated Trypsin, LysC, and Chymotrypsin-Trypsin digested samples were primarily, or solely, of HEK293 origin. All other portions of the data, including all non-SAX and remaining SAX digests are unaffected.
We have now acquired replacement HeLa data, and rebuilt confetti (build 1.2) using this data. The original results can still be accessed via the 'all builds' links on this site. However, by default, results shown are from the corrected 1.2 build. All contaminated and corrected datasets are annotated.
We remain in communication with the journal r.e. correcting the published work. We thank Ron Beavis for his diligence, and Hamssika Chandrasekaran (Mirzaei Lab) for her considerable efforts in preparing and running the replacement samples.
We collected over 1TB of mass-spec data from HeLa lysate.
Explore it at the gene or protein level in confetti - our multi-enzyme map of the HeLa proteome.
See our manuscript: Guo et. al., Confetti: A Multiprotease Map of the HeLa Proteome for Comprehensive Proteomics, Molecular & Cellular Proteomics, 13(6), 1573-1584. [Journal Link]
As well as a lot of lysate, enzyme, and mass-spec time we used:
Can't get coverage of a PTM of interest? Not enough good tryptic peptides for your SRM or PRM experiment? Maybe confetti can help.
We identified over 7,500 proteins in HeLa lysate, which are covered by more than 375,000 unique peptide sequences.
Confetti was built by members of the Mirzaei Laboratory and Proteomics Core, UT Southwestern Medical Center in Dallas, TX:
This work was supported by:
We acknowledge the Texas Advanced Computing Center (TACC) at The University of Texas at Austin for providing HPC resources that have contributed to this project.
Copyright © 2013, 2014 UT Southwestern Medical Center, Dallas TX.