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The overall
goal of this program is to develop groundbreaking techniques in analytical
and functional proteomics and to apply these methods to better understand
sleep biology. In particular, the Center for Proteomics Research
focuses on tools to monitor and manipulate membrane receptors and
transcription factors (TFs), since these regulatory proteins are
amongst the most interesting proteins in terms of understanding complex
biological processes such as sleep homeostasis. Specifically, this
program aims to:
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Develop
general methods for the discovery of native ligands for any G protein-coupled
receptor (GPCR). Key tools in this effort will be the development
of a “GPCR chip” for functional proteomics experiments and a bioinformatics-driven
collection of candidate neuropeptides.
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Develop
protein-detecting microarrays based on synthetic capture molecules
and sandwich assays. Methods to monitor the post-translational
modification state of the protein in this format will also be developed.
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Develop
methods to monitor TFs on a proteome-wide basis and determine what
factors are operative in orexin-stimulated cells.
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Determine
the molecular meaning of “sleepy” and “awake”. There is currently
little or nothing known at the molecular level about sleep homeostasis
and we hope to employ novel proteomics-based approaches to shed
light on this issue.
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